CCK8 and colony formation assays demonstrated that knocking down RAB8A and using SAG, an activator of the Hh signaling pathway, rescued the reduced viability of prostate cancer cells caused by the overexpression of circ_0 005185, indicating that circ_0 005185 may influence cellular functions through RAB8A and the Hh signaling pathway (Figure S2D,E, Supporting Information). Here, RAB8A is linked to Familial prostate cancer.