CD4 and neoplasm: Specifically, one week after the injection of HCT116 cells into the flanks of NOD/ShiLtJGpt (NCG) mice to induce subcutaneous tumor formation, in vitro activated CD8+ T cells (1  ×  107 per mouse) or mixed CD4+ T cells (5 × 106 per mouse) and CD8+ T cells (5  ×  106 per mouse) cells from the same healthy donor were injected via the tail vein every week (Figure 4I).