Chemokines are considered to play a crucial role in differentiating CD4+ T cells into Tregs and the functional differentiation of Tregs.[26] Through the analysis of single‐cell RNA sequencing data for T cells from CRC patients in public databases, we determined that the CX3CL1‐CX3CR1 signaling pathway may be involved in inducing the TNFRSF9+ phenotype in Treg cells. The gene discussed is CX3CL1; the disease is colorectal carcinoma.