In this study, we found that upregulating DCLK1 promoted but downregulating DCLK1 suppressed the PCa stem cell-like phenotype, and further in vitro and in vivo experiments confirmed that DCLK1 promoted the tumorigenicity and stem cell-like properties of PCa cells via Hippo-YAP signaling. Here, DCLK1 is linked to posterior cortical atrophy.