Next, we distinguished melanoma cells and made further sub-clustering, resulting in six cell subgroups and marking their cell numbers: C0 TRPM1+ Melanoma cells (13923), C1 PIR+ Melanoma cells (13250), C2 PHLDA2+ Melanoma cells (12711), C3 ID2+ Melanoma cells (10944), C4 PCLAF+ Melanoma cells (7492), C5 CD74+ Melanoma cells (3380) (Figure 1A), and demonstrated the proportion of cells in 6 cell subtypes (Figure 1B). The gene discussed is TRPM1; the disease is melanoma.