The KEGG pathway analysis revealed that these genes were predominantly associated with key signaling pathways, including the PI3K/AKT signaling pathway, MAPK signaling pathway, Cell cycle, Cushing syndrome, Fanconi anemia pathway, Homologous recombination, Terpenoid backbone biosynthesis, Circadian rhythm, and Steroid biosynthesis (Fig. 4B). Here, AKT1 is linked to Cushing syndrome due to macronodular adrenal hyperplasia.