To further investigate how HIF-1α modulates CD47 protein in GBM, colony formation assays were conducted, and our findings under hypoxic conditions after 24 hours of culture indicated that the clonal capacity of U87 and U251 cells in the shRNA-NC group surpassed that of the shRNA-HIF-1α + CD47 Vector group. The gene discussed is CD47; the disease is glioblastoma.