To elucidate the signaling pathways regulated by HIF-1α in vivo, we conducted further analysis to examine the expression changes of pathway proteins in tumor tissues using Western blotting and found that compared to the shRNA-NC group, the expression level of P-PI3K/P-AKT protein in the tumor of the shRNA-HIF-1α group decreased (Figure 7A-C), while the expression level of PI3K/AKT protein did not significantly change. This evidence concerns the gene HIF1A and neoplasm.