Further experiments on HIF-1α's role in regulating CD47 expression in GBM by suppressing HIF-1α expression under hypoxic conditions demonstrated that compared to the shRNA-NC group, the expression of HIF-1α in U87 and U251 cells within the shRNA-HIF-1α group was significantly reduced (Figure 4G-H). The gene discussed is CD47; the disease is glioblastoma.