In this study, we knocked down HIF-1α protein expression in GBM cells and detected changes in associated pathway proteins by WB experiments, which showed that after knocking down HIF-1α protein expression, the expression levels of the P-PI3K/P-AKT pathway, MMPs protein family pathway and SOX2/OCT4 pathway were significantly reduced. This evidence concerns the gene POU5F1 and glioblastoma.