Furthermore, to determine the function of the HIF-1α protein in regulating CD47 in GBM, we initially used the CCK8 assay to assess GBM cell proliferation and found that under hypoxic conditions, the proliferation capacity of U87 and U251 cells in the shRNA-NC group exceeded that of the shRNA-HIF-1α + CD47 plasmid control group (CD47 Vector) after 24 hours of culture. This evidence concerns the gene CD47 and glioblastoma.