In total, three types of p53 staining patterns were identified: Wild-type, as heterogeneous nuclear positivity with variable staining intensity (Fig. 1A); >90% of diffuse positivity with strong nuclear staining as the overexpression type (Fig.1B); and rare staining, with <10% of tumor cells being stained; this was regarded as an absence pattern (Fig. 1C). Here, TP53 is linked to neoplasm.