Expression of H3.3(S31A) in endothelial cells in vivo using adeno-associated virus 2 (AAV2) (Extended Data Fig. 5c–e) led to a reduced Vcam1 expression in endothelial cells of the inner curvature of the aortic arch (Fig. 4j) and reduced inflammation and neointima area after partial carotid artery ligation—a model for acute disturbed-flow-induced endothelial dysfunction and atherosclerosis (Fig. 4k and Extended Data Fig. 5f,g). Here, VCAM1 is linked to endothelial dysfunction.