In total, 2,470 DEGs were identified in the C9-ALS neurospheres following a pairwise comparison to the non-ALS donor, hereafter referred to as wild-type (>1.25 FC; FDR P < 0.01; Supplementary Fig. 11a and Supplementary Data 6 and 7), with a GO and BP term analysis found to reveal enrichment for several functions previously found to be affected in induced motor neurons (iMNs) from a C9-ALS patient, including synapse organization and neurotransmitter transport66 (Supplementary Fig. 11b and Supplementary Data 8). Here, C9 is linked to amyotrophic lateral sclerosis.