To understand the role of RNF40 in hypertension‐induced cerebrovascular endothelial cell damage, we used pAAV‐hFLT1‐MCS‐EGFP‐3×Flag‐mir30shRnf40 to establish an Rnf40‐deficient model in spontaneously hypertensive rats (SHRs). This evidence concerns the gene RNF40 and Hypertension.