To study sprouting of EC on the MFC, EC control or EC PSMA were seeded in the lower channel and were perfused with regular medium, while the opposite channel remained unseeded and void of cells for perfusion with either regular medium or medium supplemented with tumor promoting phorbol 12-myristate 13-acetate (PMA) and increased concentration of growth factors: Vascular Endothelial Growth Factor (VEGF) and Fibroblast Growth Factor (FGF) (enhanced medium) (Table 1). Here, VEGFA is linked to neoplasm.