To assess the effect of R428 inhibition on the interaction between Reg-TAMs and TICs, EpCAM+Tom+ TICs and Reg-TAMs from ICC-bearing Krt19-DreERAxin2-creERR26-Ai66 mice were sorted and cocultured with or without R428. This evidence concerns the gene EPCAM and intrahepatic cholangiocarcinoma.