AKT1 and intrahepatic cholangiocarcinoma: To investigate the tumor ecosystem and molecular signature in ICC, we performed hydrodynamic tail vein injection of HA-tagged AKT serine/threonine kinase (AKT) and YAP-S127A (plasmid encoding a mutant form of YAP with a mutation at S127A with Sleeping Beauty (SB) plasmids into 6- to 8-week-old C57BL/6 mice (WT) (9, 10) (Figure 1A).