To investigate TIC-derived GAS6 suppression of tumor immunity via AXL/MERTK on Reg-TAMs, we used Krt19-DreER Axin2-creER R26-Ai66 mice for AKT/YAP/SB hydrodynamic injection and sorted Reg-TAMs and epithelial cell adhesion molecule+/Tom+ (EpCAM+Tom+) TICs. Here, AKT1 is linked to neoplasm.