NACC1 and melanoma: Tregs were isolated from the SPL, LNs, and tumors of the B16-F10 melanoma–bearing NAC1-KO mice or WT mice, and the expression of CD36 on WT and NAC1-KO iTregs was analyzed at 24 hours, 48 hours, and 72 hours following treatment with 10 mM of LA.