Our study found that Tregs with NAC1 deficiency are metabolically more robust and functionally stronger than WT Tregs in the TME, with upregulation of the CD36/peroxisome proliferator–activated receptor gamma coactivator 1-alpha (PGC-1α) pathway, and promote tumor growth by increasing the infiltration of FoxP3+ Tregs into tumors. Here, NACC1 is linked to neoplasm.