NACC1 and melanoma: To further substantiate the role NAC1-deficient Tregs and their suppressive function in tumor progression, we conducted an adoptive transfer experiment in which the WT iTregs or NAC1-KO iTregs (Thy1.2+) were transferred into Thy1.1+ congenic recipient mice on day 1, and on the following day, B16-F10 melanoma cells were subcutaneously injected into the flank of the recipient mice (Figure 7A).