Further, the expression of Granzyme B (GzmB), an enzyme that is highly expressed in tumor-infiltrating Tregs (18) and plays an important role in increasing the metastatic burden in the lungs and eliminating Tconv cells in colorectal cancer (19, 20), was significantly higher in NAC1-KO iTregs than in WT iTregs in the control media, LA-containing media, or CM (Figure 3, C and D, and Supplemental Figure 3B), suggesting that GzmB is an important mediator of the suppressive capacity of NAC1-KO Tregs. This evidence concerns the gene NACC1 and neoplasm.