In addition, in experimental studies, we demonstrated decreased expression of IRIS-1 and 2 in response to hyperglycemia, and a rescuing effect of the two CD59 splice variants on insulin secretion in CD59, IRIS-1 and 2 knockout cells, suggesting a critical role of IRIS-1 and 2 variants in SNARE-dependent release of vesicle content. The gene discussed is CD59; the disease is Hyperglycemia.