C3AR1 and metabolic dysfunction-associated steatohepatitis: To determine the consequences of macrophage and Kupffer cell ablation of C3aR1, we use a murine dietary model of MALFD/MASH, the Gubra Amylin Nash (GAN) diet, which has macronutrient similarities to the Western diet and produces similar histologic and transcriptomic changes to human MASLD/MASH (Boland et al., 2019; Hansen et al., 2020; Vacca et al., 2024).