To query the impact of AlaRS functions on the proteome and lactylation state during infection with HCMV, we used two parallel approaches: (i) treatment of wild-type (WT) fibroblasts with 100 mM alanine compared to untreated cells and (ii) AARS1 KD compared to a scramble control (Fig. 3E). Here, AARS1 is linked to infection.