HNRNPK and infection: Therefore, to investigate whether modification of the hnRNPK–vRNA interaction decreases the propensity of hnRNP K to interact with viral sgRNA, we performed hnRNP K antibody-mediated coimmunoprecipitation experiments by pulling-down hnRNP K or antibody isotype control protein from CHIKV 181/25, ΔhnRNPK-BS1, or mock-infected C8-D1A murine astrocytes at 24 h post-infection (HPI).