HPSE2 and infection: When we treated our cells with OGT 2115 to inhibit HPSE1 followed by infection with HSV-2 strain 333 for 24 h, we observed a reduction in HS staining in the non-infected, HPSE2-silenced cells and negligible differences between the PcDNA control and siHPSE2 in the infected conditions (Figure 4F).