KRAS and infection: Using RT-qPCR for absolute quantitation of the NS3 gene in the supernatants of cell culture media at different infection time points (24, 36, and 48 hpi), the obtained standard curve was y = −1.408ln(x) + 40.902 with an R2 of 0.9908 (where y represents the CT value, x denotes the quantity in copies/μL, and R2 is the coefficient of determination).