Previous studies showed that TB vaccines encapsulating plasmid DNA and immunostimulants in PLGA nanoparticles induce higher levels of interferon-gamma (IFN-γ) and immunoglobulin (Ig) G2a antibodies in mice via injection compared to naked plasmid-DNA vaccines, indicating a strong Th1 polarization-directed response [129,130]. Here, IFNG is linked to tuberculosis.