Collectively, these findings indicate that Fer-1 can block the ferroptotic effects induced by HC-GO and that this process is closely related to the System Xc−/GSH/GPX4 axis, thereby confirming that HC-GO inhibits CRC cells through ferroptosis mediated by the System Xc−/GSH/GPX4 axis. Here, GPX4 is linked to colorectal carcinoma.