The mice fed a 16-week HFD to induce obesity exhibited significantly lower expression of eNOS in the endothelium (control: 0.20 ± 0.04 PI vs. HFD: 0.71 ± 0.05 PI, p < 0.0001) (Figure 6A,B,H) and media (control: 1.12 ± 0.01 PI vs. HFD: 0.39 ± 0.14 PI, p = 0.0012) (Figure 6A,B,I) of the abdominal aorta when compared to the control mice, fed a standard chow diet. Here, NOS3 is linked to obesity due to melanocortin 4 receptor deficiency.