Under optimized conditions, the analytical working range was 2.98 × 103–22.127 × 10−3 CFU/mL HP using square wave (SW) stripping voltammetry, precision of R2 = 0.9857 ± 0.0005 (SWSV), the detection limit approached to 2.5 × 102 CFU/mL HP (S/N = 3).The developed techniques have been applied to diagnosis of early-stage HP infections using stomach tissue from healthy humans and gastric patients. The gene discussed is HP; the disease is infection.