Moreover, in CUMS-induced mice, the ratios of phosphorylated PI3K (p-PI3K) to total PI3K and phosphorylated Akt (p-Akt) to total Akt are decreased, indicating that the PI3K/Akt signaling pathway plays a crucial role in regulating depression induced by chronic stress [28]. The gene discussed is AKT1; the disease is major depressive disorder.