The antitumor effect of Acantamoeba on NB cells has been previously studied, revealing that (i) antigens from lysate trophozoites induce apoptosis in cultured NB cells [55] and (ii) in co-culture experiments involving trophozoites and NB cells, AS trophozoites (T4) attacked the NB cell monolayers immediately upon addition, penetrated the monolayers, and induced apoptosis in NB cells via the Bax pathway [57]. Here, BAX is linked to neuroblastoma.