THRA and neuroblastoma: After live-cell imaging, we extracted and quantified the RNA levels of RFP (THRA1 proxy) and EGFP (THRA2 proxy) by RT-qPCR and compared them with the RNA levels of exon 9b (THRA1 specific) and exon 10 (THRA2 specific) in naïve, untransfected COS1 and N2A neuroblastoma cells, respectively (Figure 3e,f).