In the present work, we focused on the effects of two natural ITCs: aromatic benzyl isothiocyanate (BITC, mainly found in broccoli, cabbage, and watercress) and sulforaphane (SFN, mainly found in broccoli, cabbage, cauliflower, and kale), on P-gp-negative SKM-1 human AML cells and their P-gp-positive variants, SKM/VCR. This evidence concerns the gene PGP and acute myeloid leukemia.