The addition of G415R (but not rPKM1 or vehicle) into the culture medium reduced PTEN in the cells (Figure 6A,B), suggesting that EcPKM2 might play a role in downregulation of PTEN in cardiomyocyte during myocardial infarction, which subsequently protects cardiomyocytes from apoptosis and promotes proliferation. Here, PTEN is linked to myocardial infarction.