Through two-dimensional gel electrophoresis (2-DE) and Western blotting, they identified 18 distinct autoantigens in RA, including five key proteins: vimentin, gelsolin, alpha 2 HS glycoprotein (AHSG), glial fibrillary acidic protein (GFAP), and a1B-glycoprotein (A1BG), which showed significantly higher expression in RA SF than in the controls [107]. This evidence concerns the gene GSN and rheumatoid arthritis.