Our detection of DAMP emission from SpiD3-treated CLL cell lines substantiates the capacity of CLL cells to propagate ICD by: (i) conferring “eat me” signals to engage immune cells; and (ii) establishing a chemotactic gradient for immune cell recruitment through the release of ATP and HMGB1 into the extracellular space. This evidence concerns the gene HMGB1 and B-cell chronic lymphocytic leukemia.