OSU-CLL cells display enhanced CALR surface expression at both 24 h and 48 h timepoints in an SpiD3 dose-dependent manner (Figure 1C,D), with CALR translocation induced most prominently by cells treated with 2 μM SpiD3 for 24 h (Figure 1C). Here, CALR is linked to B-cell chronic lymphocytic leukemia.