The HG-3 CLL cell line demonstrated intense ecto-CALR expression following 24 h of treatment, starting at the 1 μM SpiD3 concentration and emboldened by the 2 μM SpiD3 concentration, in a manner comparable to that of the positive control, etoposide (Figure 1A). This evidence concerns the gene CALR and B-cell chronic lymphocytic leukemia.