Interestingly, OSU-CLL cells appeared to release HMGB1 more readily than HG-3 cells, which was demonstrated by the greater magnitude of HMGB1-associated luminescence detected from OSU-CLL cells treated with 0.5 μM or 1 μM SpiD3, compared to their HG-3 cell counterparts (Figure 3). This evidence concerns the gene HMGB1 and B-cell chronic lymphocytic leukemia.