MGMT and anaplastic astrocytoma: The methylation status of the promoter of the gene that codes for the MGMT protein was searched by polymerase chain reaction (PCR) in 18 patients, finding methylation in 3/10 glioblastomas (30%), 2/3 anaplastic astrocytoma (66%), and 4/4 tumors of the oligodendroglial lineage (100%) (Supplementary Materials S1).