When macrophages were infected with a high dose of DENV-1 (MOI = 1), NLRP3 and GSDMD mRNA reached a peak value in the early stage of infection (h.p.i = 24 h), while the Caspase-1 mRNA level gradually increased with the infection time (Figure 3A–C). The gene discussed is GSDMD; the disease is infection.