The results demonstrated that in the livers of db/db mice, hepatic PTGS2 expression was elevated, hepatocytes exhibited extensive degeneration with globular changes, hepatocyte volume was increased, several different sizes of fat particles were observed in hepatocytes, there were inflammatory cells infiltrating in the portal area, the normal structure of the hepatic lobules was damaged, and the hepatic cords were disorganized, with the pathological manifestations of MASLD. This evidence concerns the gene PTGS2 and metabolic dysfunction-associated steatotic liver disease.