Consistent with the results from the scRNA-seq data and fresh BM samples with no stimulation, TIM-3+ NK cells had higher intracellular PFP and GZMB levels than TIM-3− NK cells in AML patients’ BMMCs after in vitro stimulation (p = 0.047 and 0.078), and HDs showed similar trends (both p = 0.062, Figure 3A,B). The gene discussed is PRF1; the disease is acute myeloid leukemia.