In accordance with total NK cells, PFP and GZMB levels exhibited a positively correlated tendency with K562 cell apoptosis in both TIM-3+ and TIM-3− NK cells in AML patients, and these correlations were more significant in TIM-3+ than in TIM-3− NK cells (TIM-3+: r = 0.89, p = 0.019; r = 0.79, p = 0.063; TIM-3−: r = 0.72, p = 0.11; r = 0.70, p = 0.12, Figure 4E,F,H,I). The gene discussed is PRF1; the disease is acute myeloid leukemia.