The expression patterns of NK cell TIM-3, PFP and GZMB in fresh BM samples of AML patients and HDs tested by MFC are shown in Figure 2A. The TIM-3 and PFP levels of NK cells were similar between AML patients and HDs (median (range): 68.9% (19.7–95.0%) vs. 68.4% (41.5–85.0%), p = 0.99; 94.1% (40.5–99.4%) vs. 92.7% (84.7–96.3%), p = 0.43), whereas AML patients had significantly lower GZMB levels than HDs (median (range): 79.5% (17.3–95.7%) vs. 88.8% (79.6–94.9%), p = 0.026). The gene discussed is PRF1; the disease is acute myeloid leukemia.