In this study, we analyzed the role of the regulatory axis composed of circ_0079593 and miR-516b-5p in two types of melanoma metastasis cell lines (LM-36 and LM-16), with a focus on their interaction with the mRNAs of MCAM and CHAF1B, both at high expression levels associated with an aggressive phenotype [32,33,34]. The gene discussed is CHAF1B; the disease is melanoma.