ISCA1 methylation was negatively correlated with at least five immune gene set scores in BLCA and PAAD but positively correlated in COAD, DLBC, GBM, HNSC, KIRC, LIHC, LUAD, LUSC, MESO, SKCM, and TGCT (Supplementary Figure S11B). Here, ISCA1 is linked to bladder transitional cell carcinoma.