ISCA1 methylation was negatively related to six tumor stemness features in COAD, DLBC, HNSC, KIRP, LIHC, LUAD, LUSC, and TGCT and positively related to DMPs, DNAss, ENHss, and EREG.METH was negatively related to BLCA, LGG, and PAAD but negatively related to CESC, ESCA, KIRC, LIHC, PCPG, SARC, and SKCM (Supplementary Figure S15C). The gene discussed is EREG; the disease is neoplasm.