The results revealed that ISCA1 CNV was positively correlated with TGFBR1 RNA expression in most cancers (Supplementary Figure S12B), and ISCA1 methylation was correlated with the RNA expression levels of these 33 immune genes in COAD, LIHC, and TGCT (Supplementary Figure S12C). This evidence concerns the gene ISCA1 and cancer.