In addition, central carbon metabolism in cancer (KEGG:05230), the biosynthesis of amino acids (KEGG:01230) and metabolic pathways (KEGG:01100) were identified as being significantly downregulated, as shown in Figure 4C. Further KEGG pathway analysis, using the more stringent adjusted p-value (AdjP), identified DNA replication and the cell cycle pathway to be significantly enriched, which highlights their sensitivity in the response to nucleolin depletion. The gene discussed is NUCLEOLIN; the disease is cancer.