TNF and infection: The TNF-α mRNA levels were observed to increase by 4.8 (p < 0.05), 12.4 (p < 0.001) and 12.1 (p < 0.001)-fold on average at days 10, 20 and 30 post-infection with T. gondii, respectively, in comparison to the healthy control groups (Figure 10 and Table 3).