The results of anti-CD4 and CD8 staining of tumor tissues demonstrated that compared with the PBS and DTT control group, inoculation of DTT-EG3, DTT-EG5, DTT-EG6, and DTT-EG7 vaccines led to a significant increase in the proportion of CD4+ T cells in tumor tissues (Figure 6C, Supplementary Table S4). This evidence concerns the gene CD8A and neoplasm.