Three types of phagocytosis assays were employed to evaluate the effects and mechanisms of mTOR blockade on tumor cell phagocytosis: (1) flow-cytometry- and (2) microscopy-based phagocytosis assays, to assess the phagocytic affinity by quantifying the macrophages capable of engulfing tumor cells, and (3) luminescence-based phagocytosis assays, to assess the phagocytic capacity by quantifying the tumor cells that survived phagocytosis. This evidence concerns the gene MTOR and neoplasm.