CASP3 and neoplasm: In this study, we used the zebrafish xenograft assay to investigate the drug responses of the transplanted group 3 cell lines to BRQ, and found significantly inhibited tumour growth and an increase in Caspase-3 positive cells in D458 xenografts compared to vehicle (DMSO)-treated controls (Figure 4D,E and Figure S3B–D) after 72 h of exposure.