Human breast cell lines used in this study were as follows: MDA-MB-468, which are highly invasive adenocarcinoma triple-negative cells (ER−, PR−, Her2−); MCF-7, which are noninvasive adenocarcinoma ER positive (ER+) cells; and MCF-12A, which are non-tumorigenic ER-negative (ER−), mimicking normal breast cells. Here, ESR1 is linked to adenocarcinoma.