Hence, the present study aimed to analyze genes associated with the immune system such as TNFAIP6, IFRD1, IFITM2, IFNGR1, IRF6, and NFIL3 in cell lines such as control MCF-10F (Ct), a triple-negative breast cancer cell line, estrogen (E), Alpha3 (A3), Alpha5 (A5), and Tumor2 (T2). Here, NFIL3 is linked to triple-negative breast carcinoma.