AIF1 and neoplasm: To expand the knowledge on the immune contexture in ASACs, the extent and distribution of TILs and TAMs, CD3+ lymphocytes, CD20+ B lymphocytes, FoxP3+ T regulatory lymphocytes, and Iba-1+ macrophages were analyzed at the invasive margin and in the center of the tumor, and the results were correlated with clinical variables of tumor size, metastatic status, and clinical stage at the time of diagnosis.