Therefore, we analyzed the ability of SPION‐loaded CAR‐T cells to bind and lyse their target cells CAR‐T cells were coincubated with cell lines varying CSPG4 expression levels, namely human embryonic kidney cells (293T) and submaxillary salivary gland carcinoma cells (A253) with no/low expression, conjunctival melanoma cells (CRMM2) and uveal melanoma cells (UPMM3) with medium expression, and amelanotic melanoma cells (A375M) expressing high quantities of CSGP4 (Figures 2A,B and S2A). The gene discussed is CSPG4; the disease is melanoma.