The results showed that female age, basal serum LH and testosterone, AMH, infertility factors, PCOS, Gn starting dose, Gn total dose, E2 level on trigger day, the number of follicles ≥14 mm but <16 mm, and MII oocytes did not enter the regression equation (P > 0.05), indicating that they are not independent factors influencing polyspermy. This evidence concerns the gene PLOD1 and polycystic ovary syndrome.