Therefore, we generated multiple human lung adenocarcinoma A549 clonal cell lines depleted for ZAP, KHNYN, or both by CRISPR/Cas9 genome editing as well as a vector control (Vector) and a negative control guide targeting E. coli LacZ beta-galactosidase (LacZ) clonal cell lines (Fig 3A–B). Here, ZC3HAV1 is linked to lung adenocarcinoma.