Flow cytometry analysis of immune cells from the tumor tissues revealed that squalene could reduce the percentage of macrophages (CD11b+ F4/80+) in the CD45+ cell population (Figure 3D) and also decreased the proportion of M2 (CD206+) macrophages (Figure 3E), similar to that observed in the SQLE-KD experiments. The gene discussed is PTPRC; the disease is neoplasm.