TOMM20 and Hyperglycemia: Given that our STZ-treated mice have robust and chronic hyperglycemia, we analyzed mitochondrial number and distribution in the MHb with immunofluorescent staining for the outer mitochondrial membrane complex subunit 20 (TOM-20) at 6- and 12-week endpoints focusing our fields of view (FOV) on the ventral MHb (vMHb) where cholinergic neurons are present or the central LHb (Figures 2A,B).