To examine the possible effect of the mutation at the molecular level, we performed computational modeling and simulations of LYNX2 binding to the α7:α7 interfaces of α7 nAChR (Figure 2), a common nAChR subtype expressed in anxiety pathways of rodents and shown to be critical in regulating amygdala excitability (Klein and Yakel, 2006; Pidoplichko et al., 2013; Almeida-Suhett et al., 2014; Jiang et al., 2016). Here, CHRNA4 is linked to Anxiety.